| 1. | Pbi121 was chosen as binary vector
构建了dreb1c正义植物双元表达载体。 |
| 2. | Finally this fusion was inserted into the binary vector pbinplus . named pbgsb
插入植物双元表达载体pbinplus中，构建得到brazzein基因的表达载体pbgsb 。 |
| 3. | Finally the expression cassette of the two genes were inserted into binary vector pbin19
最后得到的植物表达载体pbm含目的基因及植物选择标记基因npt 。 |
| 4. | From the mapping relationship between information vector matrix and relative vectors , rough approximation , attribute relative reductions and selecting optimal attribute reductions set algorithms based on binary vector matrix were proposed
摘要根据向量矩阵与向量之间的映射关系，研究了基于二元向量矩阵算法的粗糙近似、属性约简以及最优属性约简集的获取。 |
| 5. | They indicated that ecbp21 may have some relative with the response to stress treatments . further more , we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method
4 .初步开展了转基因研究:为了进一步确定ecbp21在植物生长发育过程中的作用，构建了ecbp21植物表达二元载体，通过真空渗透法转化了拟南芥并获得了转基因植株。 |
| 6. | The modified sequence was cloned into binary vector pbi121 . the constructed expression vector was named pbi121 - bar , then transferred into agrobacterium tumefaciens lba4404 by triparental crossing . thus the project bacterium has been successfully constructed
改造后的目的基因克隆于真核表达载体pbi121 ，构建表达载体pbi121 - bar ，通过三亲杂交法将真核表达载体pbi121 - bar转入农杆菌lba4404 ，成功构建出工程菌。 |
| 7. | The expression of tstrx from different tissue also shows different . these results show that this gene is a stress response gene or plays an important role in slat stress tolerance . aiming to identify the functions of this gene and do some further study , we have cloned the gene into the agrobacterium tumefaciens binary vector prok ii and pcambia 3013
结合实验结果以及用200mmol . l ~ ( - 1 ) nacl处理的小盐芥地上部分构建的zap - cdna文库中得到的编码硫氧还蛋白的cdna序列的相关资料推测： tstrx基因可能在保护盐芥免受因胁迫引起的氧化损伤中起作用。 |
